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^...Murphy,...K.;...Berg,...K.;...Eshleman,...J....Contents...1...Method...1.1...Dye-terminator...sequencing...1.2...Automation...and...sample...preparation...1.3...Challenges...2...Microfluidic...Sanger...sequencing...2.1...Applications...of...microfluidic...sequencing...technologies...2.2...Device...design...2.3...Sequencing...chemistry...2.4...Platforms...2.5...Comparisons...to...other...sequencing...techniques...3...References...4...Further...reading...5...External...links.......This....problem....has....been....addressed....with....the....use....of....modified....DNA....polymerase....enzyme....systems....and....dyes....that....minimize....incorporation....variability,....as....well....as....methods....for....eliminating...."dye....blobs".....Comparisons..to..other..sequencing..techniques[edit]...high....consumption....of....expensive....reagents,....reliance....on....expensive....equipment,....personnel-intensive....manipulations,....etc.)....by....integrating....and....automating....the....Sanger....sequencing....steps.....MBI....Says....New....Tool....That....Automates....Sanger....Sample....Prep....Cuts....Reagent....and....Labor....Costs.........Cookies...are...required...to...use...this...site....Three-dimensional...channel...interconnections...and...microvalves...are...formed...by...the...PDMS...and...bottom...manifold...glass...wafer....

Genomics.....13...(7):...2399412....These...programs...score...the...quality...of...each...peak...and...remove...low-quality...base...peaks...(which...are...generally...located...at...the...ends...of...the...sequence)....The....DNA....sample....is....divided....into....four....separate....sequencing....reactions,....containing....all....four....of....the....standard....deoxynucleotides....(dATP,....dGTP,....dCTP....and....dTTP)....and....the....DNA....polymerase.....The....reaction....mixtures....are....combined....and....co-electrophoresed....down....a....single....polyacrylamide....gel....tube,....the....separated....fluorescent....bands....of....DNA....are....detected....near....the....bottom....of....the....tube,....and....the....sequence....information....is....acquired....directly....by....computer....."DNA...sequencing...and...genotyping...in...miniaturized...electrophoresis...systems"....Sequencing..reactions..(thermocycling..and..labelling),..cleanup..and..re-suspension..of..samples..in..a..buffer..solution..are..performed..separately,..before..loading..samples..onto..the..sequencer...(1986).....

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Sam 3 Sep - 18:33 (2016)
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